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相关教师 |
万俊，副教授，苏州大学唐仲英血液学研究中心
办公室地址：苏州市仁爱路199号，苏州大学独墅湖校区一期703楼 B614室，邮编215123
实验室地址：苏州市仁爱路199号，苏州大学独墅湖校区一期703楼3618室 (戴克胜教授团队)
联系邮箱：jwan@suda.edu.cn
教育经历
2009.9 – 2013.6；湖南大学生物学院，学士
2013.9 – 2016.6；湖南大学生物学院，硕士(导师：王柯敏教授、郭秋平教授)
2016.10 – 2021.6；荷兰马斯特里赫特大学，博士(导师：Tilman Hackeng，Mark Roest，Bas de Laat)
工作经历
2021.10 – 2022.10；美国北卡罗来纳大学教堂山分校，博士后 (导师：Nigel Mackman)
2023.1 –今； 苏州大学唐仲英血液学研究中心，副教授，硕士研究生导师
研究方向: 血栓与止血
（1）细胞对凝血系统的调控机制，及其在重大疾病中的作用
探索血液循环中的细胞（血小板、红细胞、白细胞、胞外囊泡、癌细胞等）对凝血级联系统以及纤溶系统的新调控机制，以及此类调控机制在重大疾病如心血管疾病和癌症中的作用。
（2）新型凝血功能检测法的开发和应用
凝血酶生成检测法 (Thrombin generation assay, TGA)是目前最广泛应用的新型凝血功能检测法，它能提供比传统凝血检测法(PT、APTT)更高的灵敏度。本人已成功开发新型全血凝血酶生成检测法(全血TGA)，可对人和鼠全血样本的凝血潜能进行高灵敏度的直接量化检测。 全血TGA的优点包括：(1) 直接检测全血样本，省去了对样本进行预处理的时间，有潜力开发成为床旁检测法(POCT)；(2) 全血TGA相比血浆TGA的灵敏度更高：能更灵敏的检测出小鼠凝血因子IX, XI, PKa 和C1-INH的敲除对凝血系统的影响。 全血TGA的潜在应用领域: (1)作为研究红细胞等血细胞对凝血过程的调控机制的新工具, 为临床血栓/出血疾病提供机制解释；(2)高灵敏度地筛选新型抗栓/止血药物(有效性和特异性); (3)优化/指导临床抗栓/止血药物的剂量; (4)作为指示临床血栓/出血风险的POCT。
主持科研项目
2023.01 - 2027.12；苏州大学优秀青年学者(副教授)科研启动经费，主持；
2024.01 - 2027.12; 国家自然科学基金 面上项目；#82370137，主持；
2023.07 - 2025.06；江苏省高等学校基础科学（自然科学）研究面上项目；主持；
论文发表
（共同）第一作者或通讯作者 (#共同第一作者, *通讯作者)
1)Jun Wan, Sophia Dhrolia, Rohan R Kasthuri, Yuriy Prokopenko, Anton Ilich, Prakash Saha, Mark Roest, Alisa S. Wolberg, Nigel S Key, Rafal Pawlinski, Pavan K Bendapudi, Nigel Mackman and Steven P Grover. Plasma kallikrein supports FXII-independent thrombin generation in mouse whole blood. Blood Advances. 2024; (IF2024=7.4)
2)Jun Wan, Pansakorn Tanratana, Mark Roest, Andras Gruber, Rafal Pawlinski, Alisa S Wolberg, Nigel Mackman and Steven P Grover. A novel mouse whole blood thrombin generation assay sensitive to FXI- and FIX-mediated amplification of coagulation. Blood Advances. 2023; 7(9): 1915-25. May 9 (IF2023=7.5)
3)Jun Wan, Nadira Vadaq, Joke Konings, Martin Jaeger, Vinod Kumar, Bas de Laat, Leo Joosten, Mihai G Netea, Andre J van der Ven, Philip G de Groot, Quirijn de Mast and Mark Roest. Kallikrein augments the anticoagulant function of the protein C system in thrombin generation. Journal of Thrombosis and Haemostasis. 2022; 20(1): 48-57. Jan (IF2022=10.4)
4)Jun Wan *, Joke Konings, Bas de Laat, Tilman M Hackeng and Mark Roest. Added Value of Blood Cells in Thrombin Generation Testing. Thrombosis and Haemostasis. 2021; 121(12): 1574-87. Dec (IF2021=6.681)
5)Lie Li#, Jun Wan# (co-first), Xiaohong Wen, Qiuping Guo, Huishan Jiang, Jie Wang, Yazhou Ren and Kemin Wang. Identification of a New DNA Aptamer by Tissue-SELEX for Cancer Recognition and Imaging. Analytical Chemistry. 2021; 93(19): 7369-77. May 18 (IF2021= 8.008)
6)Jun Wan, Lara N Roberts, Wasiliki Hendrix, Joke Konings, Tsai-Wing Ow, Liane Rabinowich, Omar Barbouti, Bas de Laat, Roopen Arya, Vishal C Patel, Mark Roest, Ton Lisman and William Bernal. Whole blood thrombin generation profiles of patients with cirrhosis explored with a near patient assay. Journal of Thrombosis and Haemostasis. 2020; 18(4): 834-43. 2020-04-01 (IF2020=5.824)
7)Jun Wan, Joke Konings, Qiuting Yan, Hilde Kelchtermans, Romy Kremers, Bas de Laat and Mark Roest. A novel assay for studying the involvement of blood cells in whole blood thrombin generation. Journal of Thrombosis and Haemostasis. 2020; 18(6): 1291-301. 2020-06-28 (IF2020=5.824)
8)Jun Wan, Ling Ye, Xiaohai Yang, Qiuping Guo, Kemin Wang, Zhixiang Huang, Yuyu Tan, Baoyin Yuan and Qin Xie. Cell-SELEX based selection and optimization of DNA aptamers for specific recognition of human cholangiocarcinoma QBC-939 cells. Analyst. 2015; 140(17): 5992-7. Sep 7 (IF2015=4.033)
其他参与发表论文
1)F Meng, S Chen, C Liu, MS Khan, Y Yan, J Wan, Y Xia, C Sun, et al. The role of PKC in X-ray-induced megakaryocyte apoptosis and thrombocytopenia. Blood Cells Mol Dis. 2024; 104: 102798.
2)S Sun, E Campello, J Zou, J Konings, D Huskens, J Wan, DI Fernández, C Reutelingsperger, et al. Crucial roles of red blood cells and platelets in whole blood thrombin generation. Blood Adv. 2023; 7: 6717-31.
3)SP Grover, T Kawano, J Wan, P Tanratana, Z Polai, YJ Shim, O Snir, S Braekkan, et al. C1 inhibitor deficiency enhances contact pathway-mediated activation of coagulation and venous thrombosis. Blood. 2023; 141: 2390-401.
4)GS Temba, N Vadaq, J Wan, V Kullaya, D Huskens, T Pecht, M Jaeger, CK Boahen, et al. Differences in thrombin and plasmin generation potential between East African and Western European adults: The role of genetic and non-genetic factors. J Thromb Haemost. 2022; 20: 1089-105.
5)L Li, M Roest, Y Sang, JA Remijn, R Fijnheer, K Smit, D Huskens, J Wan, et al. Patients With Multiple Myeloma Have a Disbalanced Whole Blood Thrombin Generation Profile. Front Cardiovasc Med. 2022; 9: 919495.
6)S Huang, W van der Heijden, IJ Reuling, J Wan, Q Yan, RMW de Laat-Kremers, AJ Van der Ven, PG de Groot, et al. Functional changes in hemostasis during asexual and sexual parasitemia in a controlled human malaria infection. PLoS One. 2022; 17: e0271527.
7)WA van der Heijden, J Wan, L Van de Wijer, M Jaeger, MG Netea, AJ van der Ven, PG de Groot, M Roest, et al. Plasmatic Coagulation Capacity Correlates With Inflammation and Abacavir Use During Chronic HIV Infection. J Acquir Immune Defic Syndr. 2021; 87: 711-9.
8)ZX Huang, Q Xie, QP Guo, KM Wang, XX Meng, BY Yuan, J Wan and YY Chen. DNA aptamer selected for specific recognition of prostate cancer cells and clinical tissues. Chin Chem Lett. 2017; 28: 1252-7.
9)B Yuan, Y Zhou, Q Guo, K Wang, X Yang, X Meng, J Wan, Y Tan, et al. A signal-on split aptasensor for highly sensitive and specific detection of tumor cells based on FRET. Chem Commun (Camb). 2016; 52: 1590-3.
10)Q Xie, YY Tan, QP Guo, KM Wang, BY Yuan, J Wan and XY Zhao. A fluorescent aptasensor for sensitive detection of human hepatocellular carcinoma SMMC-7721 cells based on graphene oxide. Analytical Methods. 2014; 6: 6809-14.
11)QP Guo, XY Zhao, Q Xie, KM Wang, J Wan, BY Yuan and YY Tan. Label-free Fluorescence Signal Amplifying Detection of DNA Based on Hairpin Probe and Exo III. Chemical Journal of Chinese Universities-Chinese. 2014; 35: 1646-51.
荣誉
2025年 唐仲英基金会“仲英青年学者”
2024年 ISTH 2024 Congress, Reach-the-world travel award;
2023年 江苏省血液研究所“陈悦书医学奖 -苏州血液研究青年科技奖”
2021年 ISTH 2021 Congress, Early career travel award;
2015年 研究生国家奖学金;
学术兼职 &社会服务
国际血栓与止血学会(ISTH), 会员 2023-2025
中国病理生理学会; 中国生理学会， 会员 2023-2025；
Frontiers in Medicine, Review Editor;
多个同行评议期刊的审稿人，包括Thromb Res; RPTH; Int J Mol Sci 等
团队人员：
赵梓宇(2023级硕士生)；吕诵(2024级硕士生)；方晓晔(2024级硕士生)；杨晓雨(2025级硕士生)
已毕业学生 Alumni：
闫妍(2022级硕士生)，2025年毕业后赴 天津医科大学 读博
小组图片：
2025年6月
万俊，副教授，苏州大学唐仲英血液学研究中心
办公室地址：苏州市仁爱路199号，苏州大学独墅湖校区一期703楼 B614室，邮编215123
实验室地址：苏州市仁爱路199号，苏州大学独墅湖校区一期703楼3618室 (戴克胜教授团队)
联系邮箱：jwan@suda.edu.cn
教育经历
2009.9 – 2013.6；湖南大学生物学院，学士
2013.9 – 2016.6；湖南大学生物学院，硕士(导师：王柯敏教授、郭秋平教授)
2016.10 – 2021.6；荷兰马斯特里赫特大学，博士(导师：Tilman Hackeng，Mark Roest，Bas de Laat)
工作经历
2021.10 – 2022.10；美国北卡罗来纳大学教堂山分校，博士后 (导师：Nigel Mackman)
2023.1 –今； 苏州大学唐仲英血液学研究中心，副教授，硕士研究生导师
研究方向: 血栓与止血
（1）细胞对凝血系统的调控机制，及其在重大疾病中的作用
探索血液循环中的细胞（血小板、红细胞、白细胞、胞外囊泡、癌细胞等）对凝血级联系统以及纤溶系统的新调控机制，以及此类调控机制在重大疾病如心血管疾病和癌症中的作用。
（2）新型凝血功能检测法的开发和应用
凝血酶生成检测法 (Thrombin generation assay, TGA)是目前最广泛应用的新型凝血功能检测法，它能提供比传统凝血检测法(PT、APTT)更高的灵敏度。本人已成功开发新型全血凝血酶生成检测法(全血TGA)，可对人和鼠全血样本的凝血潜能进行高灵敏度的直接量化检测。 全血TGA的优点包括：(1) 直接检测全血样本，省去了对样本进行预处理的时间，有潜力开发成为床旁检测法(POCT)；(2) 全血TGA相比血浆TGA的灵敏度更高：能更灵敏的检测出小鼠凝血因子IX, XI, PKa 和C1-INH的敲除对凝血系统的影响。 全血TGA的潜在应用领域: (1)作为研究红细胞等血细胞对凝血过程的调控机制的新工具, 为临床血栓/出血疾病提供机制解释；(2)高灵敏度地筛选新型抗栓/止血药物(有效性和特异性); (3)优化/指导临床抗栓/止血药物的剂量; (4)作为指示临床血栓/出血风险的POCT。
主持科研项目
2023.01 - 2027.12；苏州大学优秀青年学者(副教授)科研启动经费，主持；
2024.01 - 2027.12; 国家自然科学基金 面上项目；#82370137，主持；
2023.07 - 2025.06；江苏省高等学校基础科学（自然科学）研究面上项目；主持；
论文发表
（共同）第一作者或通讯作者 (#共同第一作者, *通讯作者)
1)Jun Wan, Sophia Dhrolia, Rohan R Kasthuri, Yuriy Prokopenko, Anton Ilich, Prakash Saha, Mark Roest, Alisa S. Wolberg, Nigel S Key, Rafal Pawlinski, Pavan K Bendapudi, Nigel Mackman and Steven P Grover. Plasma kallikrein supports FXII-independent thrombin generation in mouse whole blood. Blood Advances. 2024; (IF2024=7.4)
2)Jun Wan, Pansakorn Tanratana, Mark Roest, Andras Gruber, Rafal Pawlinski, Alisa S Wolberg, Nigel Mackman and Steven P Grover. A novel mouse whole blood thrombin generation assay sensitive to FXI- and FIX-mediated amplification of coagulation. Blood Advances. 2023; 7(9): 1915-25. May 9 (IF2023=7.5)
3)Jun Wan, Nadira Vadaq, Joke Konings, Martin Jaeger, Vinod Kumar, Bas de Laat, Leo Joosten, Mihai G Netea, Andre J van der Ven, Philip G de Groot, Quirijn de Mast and Mark Roest. Kallikrein augments the anticoagulant function of the protein C system in thrombin generation. Journal of Thrombosis and Haemostasis. 2022; 20(1): 48-57. Jan (IF2022=10.4)
4)Jun Wan *, Joke Konings, Bas de Laat, Tilman M Hackeng and Mark Roest. Added Value of Blood Cells in Thrombin Generation Testing. Thrombosis and Haemostasis. 2021; 121(12): 1574-87. Dec (IF2021=6.681)
5)Lie Li#, Jun Wan# (co-first), Xiaohong Wen, Qiuping Guo, Huishan Jiang, Jie Wang, Yazhou Ren and Kemin Wang. Identification of a New DNA Aptamer by Tissue-SELEX for Cancer Recognition and Imaging. Analytical Chemistry. 2021; 93(19): 7369-77. May 18 (IF2021= 8.008)
6)Jun Wan, Lara N Roberts, Wasiliki Hendrix, Joke Konings, Tsai-Wing Ow, Liane Rabinowich, Omar Barbouti, Bas de Laat, Roopen Arya, Vishal C Patel, Mark Roest, Ton Lisman and William Bernal. Whole blood thrombin generation profiles of patients with cirrhosis explored with a near patient assay. Journal of Thrombosis and Haemostasis. 2020; 18(4): 834-43. 2020-04-01 (IF2020=5.824)
7)Jun Wan, Joke Konings, Qiuting Yan, Hilde Kelchtermans, Romy Kremers, Bas de Laat and Mark Roest. A novel assay for studying the involvement of blood cells in whole blood thrombin generation. Journal of Thrombosis and Haemostasis. 2020; 18(6): 1291-301. 2020-06-28 (IF2020=5.824)
8)Jun Wan, Ling Ye, Xiaohai Yang, Qiuping Guo, Kemin Wang, Zhixiang Huang, Yuyu Tan, Baoyin Yuan and Qin Xie. Cell-SELEX based selection and optimization of DNA aptamers for specific recognition of human cholangiocarcinoma QBC-939 cells. Analyst. 2015; 140(17): 5992-7. Sep 7 (IF2015=4.033)
其他参与发表论文
1)F Meng, S Chen, C Liu, MS Khan, Y Yan, J Wan, Y Xia, C Sun, et al. The role of PKC in X-ray-induced megakaryocyte apoptosis and thrombocytopenia. Blood Cells Mol Dis. 2024; 104: 102798.
2)S Sun, E Campello, J Zou, J Konings, D Huskens, J Wan, DI Fernández, C Reutelingsperger, et al. Crucial roles of red blood cells and platelets in whole blood thrombin generation. Blood Adv. 2023; 7: 6717-31.
3)SP Grover, T Kawano, J Wan, P Tanratana, Z Polai, YJ Shim, O Snir, S Braekkan, et al. C1 inhibitor deficiency enhances contact pathway-mediated activation of coagulation and venous thrombosis. Blood. 2023; 141: 2390-401.
4)GS Temba, N Vadaq, J Wan, V Kullaya, D Huskens, T Pecht, M Jaeger, CK Boahen, et al. Differences in thrombin and plasmin generation potential between East African and Western European adults: The role of genetic and non-genetic factors. J Thromb Haemost. 2022; 20: 1089-105.
5)L Li, M Roest, Y Sang, JA Remijn, R Fijnheer, K Smit, D Huskens, J Wan, et al. Patients With Multiple Myeloma Have a Disbalanced Whole Blood Thrombin Generation Profile. Front Cardiovasc Med. 2022; 9: 919495.
6)S Huang, W van der Heijden, IJ Reuling, J Wan, Q Yan, RMW de Laat-Kremers, AJ Van der Ven, PG de Groot, et al. Functional changes in hemostasis during asexual and sexual parasitemia in a controlled human malaria infection. PLoS One. 2022; 17: e0271527.
7)WA van der Heijden, J Wan, L Van de Wijer, M Jaeger, MG Netea, AJ van der Ven, PG de Groot, M Roest, et al. Plasmatic Coagulation Capacity Correlates With Inflammation and Abacavir Use During Chronic HIV Infection. J Acquir Immune Defic Syndr. 2021; 87: 711-9.
8)ZX Huang, Q Xie, QP Guo, KM Wang, XX Meng, BY Yuan, J Wan and YY Chen. DNA aptamer selected for specific recognition of prostate cancer cells and clinical tissues. Chin Chem Lett. 2017; 28: 1252-7.
9)B Yuan, Y Zhou, Q Guo, K Wang, X Yang, X Meng, J Wan, Y Tan, et al. A signal-on split aptasensor for highly sensitive and specific detection of tumor cells based on FRET. Chem Commun (Camb). 2016; 52: 1590-3.
10)Q Xie, YY Tan, QP Guo, KM Wang, BY Yuan, J Wan and XY Zhao. A fluorescent aptasensor for sensitive detection of human hepatocellular carcinoma SMMC-7721 cells based on graphene oxide. Analytical Methods. 2014; 6: 6809-14.
11)QP Guo, XY Zhao, Q Xie, KM Wang, J Wan, BY Yuan and YY Tan. Label-free Fluorescence Signal Amplifying Detection of DNA Based on Hairpin Probe and Exo III. Chemical Journal of Chinese Universities-Chinese. 2014; 35: 1646-51.
荣誉
2025年 唐仲英基金会“仲英青年学者”
2024年 ISTH 2024 Congress, Reach-the-world travel award;
2023年 江苏省血液研究所“陈悦书医学奖 -苏州血液研究青年科技奖”
2021年 ISTH 2021 Congress, Early career travel award;
2015年 研究生国家奖学金;
学术兼职 &社会服务
国际血栓与止血学会(ISTH), 会员 2023-2025
中国病理生理学会; 中国生理学会， 会员 2023-2025；
Frontiers in Medicine, Review Editor;
多个同行评议期刊的审稿人，包括Thromb Res; RPTH; Int J Mol Sci 等
团队人员：
赵梓宇(2023级硕士生)；吕诵(2024级硕士生)；方晓晔(2024级硕士生)；杨晓雨(2025级硕士生)
已毕业学生 Alumni：
闫妍(2022级硕士生)，2025年毕业后赴 天津医科大学 读博
小组图片：
2025年6月
