1、分子酶学
2、生物大分子的结构与功能研究主要运用分子酶学、结构生物学、生物化学等方法对多种在细胞信号通路中起重要作用、并与多种人类疾病密切相关的酶分子（如蛋白激酶和蛋白磷酸酶）展开研究，努力揭示这些酶分子执行生物学功能的分子机制。
代表性论文:
1. Wang,YL, Zhang, YY, Lu, C, Zhang, W, Deng, H, Wu, JW, Wang, J* and Wang, ZX*. Kinetic andmechanistic studies of p38alpha MAP kinase phosphorylation by MKK6. FEBS J, 286(5): 1030-1052 (2019). (*共同通讯作者)
2. Dong, J, Du, X, Wang, H, Wang, J, Lu, C, Chen, X, Zhu, Z, Luo, Z, Yu, L, Brown, AJ, Yang, H and Wu, JW. Allostericenhancement of ORP1-mediated cholesterol transport by PI(4,5)P2/PI(3,4)P2. Nat Commun, 10(1): 829 (2019).
3. Wang, H, Ma, Q, Qi, Y, Dong, J, Du, X, Rae, J, Wang, J, Wu, WF, Brown, AJ, Parton, RG, Wu, JW and Yang, H. ORP2 DeliversCholesterol to the Plasma Membrane in Exchange for Phosphatidylinositol 4,5-Bisphosphate (PI(4,5)P2). Mol Cell, 73(3): 458-473 e7(2019).
4. Zhao,YY, Mao, MW, Zhang, WJ, Wang, J, Li, HT, Yang, Y, Wang, ZX and Wu, JW. Expanding RNA binding specificity and affinityof engineered PUF domains. Nucleic Acids Res, 46(9): 4771-4782 (2018).
5. Li, SJ#, Wang, J#, Ma L#, Lu, C, Wang, J, Wu, JW and Wang, ZX. Cooperative autoinhibition and multi-levelactivation mechanisms of calcineurin. Cell Res, 26(3): 336-349 (2016). (#共同第一作者)
6. Wu, JX, Cheng, YS, Wang, J, Chen, L, Ding, M and Wu, JW. Structural insight into the mechanism of synergistic autoinhibitionof SAD kinases. Nat Commun, 6: 8953 (2015).
7. Chai, N, Li, WX, Wang, J, Wang, ZX, Yang, SM and Wu, JW. Structural basis for the Smad5 MH1 domain to recognize differentDNA sequences. Nucleic Acids Res, 43(18): 9051-9064 (2015).
8. Wang, J#, Jiang, J#, Wang, J#, Chen, L, Fan, SL, Wu, JW, Wang, X and Wang, ZX. Structural insights into thenegative regulation of BRI1 signaling by BRI1-interacting protein BKI1. Cell Res, 24(11): 1328-1341 (2014). (#共同第一作者)
9. Xin, FJ#, Wang, J#, Zhao, RQ, Wang, ZX and Wu, JW. Coordinated regulation of AMPK activity by multiple elements inthe alpha-subunit. Cell Res, 23(10): 1237-1240 (2013). (#共同第一作者)
10. Chen, L, Xin, FJ, Wang, J, Hu, J, Zhang, YY, Wan, S, Cao, LS, Lu, C, Li, P, Yan, SF, Neumann, D, Schlattner, U, Xia, B, Wang, ZX and Wu, JW. Conserved regulatory elements in AMPK. Nature, 498(7453): E8-10 (2013).
11. Cao, LS, Wang, J, Chen, Y, Deng, H, Wang, ZX and Wu, JW. Structural basis for the regulation of maternal embryonic leucinezipper kinase. Plos One, 8(7): e70031 (2013).
12. Chen, L, Wang, J, Zhang, YY, Yan, SF, Neumann, D, Schlattner, U, Wang, ZX and Wu, JW. AMP-activated protein kinaseundergoes nucleotide-dependent conformational changes. Nat Struct Mol Biol, 19(7): 716-718 (2012).
13. Wang, J, Wu, JW and Wang, ZX. Structural insights into the autoactivation mechanism ofp21-activated protein kinase. Structure, 19(12): 1752-1761 (2011).
14. Wang, J, Wu, JW and Wang, ZX. Mechanistic studies of theautoactivation of PAK2: a two-step model of cis initiation followed by transamplification. J Biol Chem, 286(4): 2689-2695 (2011).
15. Gao, X, Wang, J, Yu, DQ, Bian, F, Xie, BB, Chen, XL, Zhou, BC, Lai, LH, Wang, ZX, Wu, JW and Zhang, YZ. Structural basisfor the autoprocessing of zinc metalloproteases in the thermolysin family. Proc Natl Acad Sci U S A, 107(41): 17569-17574 (2010).
硕士生：生化与分子生物学（071010）
1、分子酶学
2、生物大分子的结构与功能研究主要运用分子酶学、结构生物学、生物化学等方法对多种在细胞信号通路中起重要作用、并与多种人类疾病密切相关的酶分子（如蛋白激酶和蛋白磷酸酶）展开研究，努力揭示这些酶分子执行生物学功能的分子机制。
代表性论文:
1. Wang,YL, Zhang, YY, Lu, C, Zhang, W, Deng, H, Wu, JW, Wang, J* and Wang, ZX*. Kinetic andmechanistic studies of p38alpha MAP kinase phosphorylation by MKK6. FEBS J, 286(5): 1030-1052 (2019). (*共同通讯作者)
2. Dong, J, Du, X, Wang, H, Wang, J, Lu, C, Chen, X, Zhu, Z, Luo, Z, Yu, L, Brown, AJ, Yang, H and Wu, JW. Allostericenhancement of ORP1-mediated cholesterol transport by PI(4,5)P2/PI(3,4)P2. Nat Commun, 10(1): 829 (2019).
3. Wang, H, Ma, Q, Qi, Y, Dong, J, Du, X, Rae, J, Wang, J, Wu, WF, Brown, AJ, Parton, RG, Wu, JW and Yang, H. ORP2 DeliversCholesterol to the Plasma Membrane in Exchange for Phosphatidylinositol 4,5-Bisphosphate (PI(4,5)P2). Mol Cell, 73(3): 458-473 e7(2019).
4. Zhao,YY, Mao, MW, Zhang, WJ, Wang, J, Li, HT, Yang, Y, Wang, ZX and Wu, JW. Expanding RNA binding specificity and affinityof engineered PUF domains. Nucleic Acids Res, 46(9): 4771-4782 (2018).
5. Li, SJ#, Wang, J#, Ma L#, Lu, C, Wang, J, Wu, JW and Wang, ZX. Cooperative autoinhibition and multi-levelactivation mechanisms of calcineurin. Cell Res, 26(3): 336-349 (2016). (#共同第一作者)
6. Wu, JX, Cheng, YS, Wang, J, Chen, L, Ding, M and Wu, JW. Structural insight into the mechanism of synergistic autoinhibitionof SAD kinases. Nat Commun, 6: 8953 (2015).
7. Chai, N, Li, WX, Wang, J, Wang, ZX, Yang, SM and Wu, JW. Structural basis for the Smad5 MH1 domain to recognize differentDNA sequences. Nucleic Acids Res, 43(18): 9051-9064 (2015).
8. Wang, J#, Jiang, J#, Wang, J#, Chen, L, Fan, SL, Wu, JW, Wang, X and Wang, ZX. Structural insights into thenegative regulation of BRI1 signaling by BRI1-interacting protein BKI1. Cell Res, 24(11): 1328-1341 (2014). (#共同第一作者)
9. Xin, FJ#, Wang, J#, Zhao, RQ, Wang, ZX and Wu, JW. Coordinated regulation of AMPK activity by multiple elements inthe alpha-subunit. Cell Res, 23(10): 1237-1240 (2013). (#共同第一作者)
10. Chen, L, Xin, FJ, Wang, J, Hu, J, Zhang, YY, Wan, S, Cao, LS, Lu, C, Li, P, Yan, SF, Neumann, D, Schlattner, U, Xia, B, Wang, ZX and Wu, JW. Conserved regulatory elements in AMPK. Nature, 498(7453): E8-10 (2013).
11. Cao, LS, Wang, J, Chen, Y, Deng, H, Wang, ZX and Wu, JW. Structural basis for the regulation of maternal embryonic leucinezipper kinase. Plos One, 8(7): e70031 (2013).
12. Chen, L, Wang, J, Zhang, YY, Yan, SF, Neumann, D, Schlattner, U, Wang, ZX and Wu, JW. AMP-activated protein kinaseundergoes nucleotide-dependent conformational changes. Nat Struct Mol Biol, 19(7): 716-718 (2012).
13. Wang, J, Wu, JW and Wang, ZX. Structural insights into the autoactivation mechanism ofp21-activated protein kinase. Structure, 19(12): 1752-1761 (2011).
14. Wang, J, Wu, JW and Wang, ZX. Mechanistic studies of theautoactivation of PAK2: a two-step model of cis initiation followed by transamplification. J Biol Chem, 286(4): 2689-2695 (2011).
15. Gao, X, Wang, J, Yu, DQ, Bian, F, Xie, BB, Chen, XL, Zhou, BC, Lai, LH, Wang, ZX, Wu, JW and Zhang, YZ. Structural basisfor the autoprocessing of zinc metalloproteases in the thermolysin family. Proc Natl Acad Sci U S A, 107(41): 17569-17574 (2010).
硕士生：生化与分子生物学（071010）