长期从事液相色谱纯化工艺及仪器研究，提出了“少即是多”极简主义科学仪器设计理念，开发了制备型二维液相色谱系统和多糖纯化仪，开发了多种皮肤病治疗活性多糖成分，以第一作者或通讯作者在Anal Chem, J Chromatogr A, J Pharm Anal, Carbohydr Polym, 色谱等领域顶级期刊上，已发表论文36篇，以第一发明人申报发明专利23项，已授权发明专利9项，参与制定国家标准1项（GB/T 44739-2024）。获中国商业联合会科学技术奖二等奖1项和中国食品工业协会科学技术奖三等奖1项。
《中草药》《Pharm Sci Adv》等期刊青年编委、世界中联中药化学专业委员会理事、江苏省化学化工学会色谱及分离科学专业委员会委员、江苏省营养学会分子营养专业委员会委员、江苏省科技副总（苏州金记食品有限公司）。
1. 多维液相色谱分离纯化设备及其方法；
2. 多糖/寡糖的纯化及构效关系研究；
3. 多糖在药物、化妆品和保健食品中应用开发；
2026年
(1) Jiang X, Xia J, Gu T, Zhang M, Wang Y, Han H, et al. Structure and bioactivities of a glycan isolated from Fritillaria unibracteata. Int J Biol Macromol. 2026;357:151613.
(2) Xia J, Zohaib MA, Su X, Ji W, Hu T, Zhou J, et al. Uncovering a photoprotective polysaccharide from Gentiana dahurica via systematic fractionation. Pharmaceutical Science Advances. 2026;4:100120.
(3) Zhao L, Gao Z, Yuan Z, Yang X, Li D, Ren Q, et al. DC-CD4 bispecific tolerogenic nanovesicles induce antigen-specific regulatory T cells and ameliorate collagen-induced arthritis in mice. Nature Communications. 2026.
(4) Li S, Wang K, Shi W, Wang X, Li D, Liu Y, et al. Vm–MSI: a Vancomycin–Antimicrobial Peptide Conjugate Combating Resistant Bacteria and Broadening the Antimicrobial Spectrum. Advanced Science. 2026;13(11):e03023.
2025年
(1) Zhu, K.; Yao, Z.; Gu, T.; Jiang, X.; Zhou, J.*; Li, D.* Study of the ability of polysaccharides isolated from Zizania latifolia to promote wound healing in mice via in vitro screening and in vivo evaluation. Food Chem 2025, 464, 141810.
(2) Ain, N. U.; Akhlaq, M. H.; Zhu, K.; Ji, W.; Yi, L.*; Li, D.*; Zhang, Z.* A nanoemulgel formulation of acetylated Crataegus pinnatifida polysaccharide for the treatment of skin photoaging in mice. Int J Biol Macromol 2025, 303, 140649.
(3) Ji W, Nie X, Sun L, Wang Y, Tian S, Li D. Development of hydro-distillation extraction and macroporous resin enrichment method for GC-MS chemical profiling of volatile components in the dried root of Gentiana dahurica Fisch. Nat Prod Res. 2025;39(20):6016–22.
(4) Li G, Shi Y, Zhu J, Zhu K, Hu B, Huang X, et al. Active Peptides Derived from Snail Mucus Promoted Wound Healing by Enhancing Endothelial Cell Proliferation and Angiogenesis. International Journal of Molecular Sciences. 2025;26(21):10341.
(5) 王子梵, 姚振, 初红涛, 许展鹏, 李笃信, 张维冰. 基于溶剂诱导相变萃取法麻类植物中糖苷与苷元的分级研究. 齐齐哈尔大学学报(自然科学版). 2025(01):2–7.
(6) Zhang M, Zhao L, Xia J, Zhu X, Liu J, Sun L, et al. Exploring the hepatoprotective efficacy of Cortex Fraxini from the perspective of interplay between antibacterial molecule and the gut microbiota. Fitoterapia. 2025;185:106737.
2024年
(1) Wang J.; Zhu, K.; Zhang, M.; Zhou, Q.; Ji, W.; Yao, Z.; Li, D*. Pharmacokinetics, tissue distribution, and subacute toxicity of oral carrageenan in mice. Int J Biol Macromol 2024, 266, 130725.
(2) Li, D.; Du, X.; Bai, W.; Schmitz, O. J. Sample preparation of Hedyotis diffusa Willd. for two-dimensional gas chromatography-time of flight mass spectroscopic analysis enhanced chemical profiling. Pharm Sci Adv 2024, 2, 100056.
(3) Ain, N. U.; Khan, B.; Zhu, K.; Ji, W.; Tian, H.; Yu, X.; Yi, L.*; Li, D.*; Zhang, Z.* Fabrication of mesoporous silica nanoparticles for releasable delivery of licorice polysaccharide at the acne site in topical application. Carbohydr Polym 2024, 339, 122250.
(4) Ji, W.; Qian, C.; Su, X.; Li, X.; Zhang, Z.; Ma, Y.*; Zhang, M.*; Li, D.* Structure characterization and protective effect against UVB irradiation of polysaccharides isolated from the plateau plant Gentiana dahurica Fisch. Int J Biol Macromol 2024, 267, 131551.
(5) Yao, Z.; Zhu, K.; Gu, T.; Schmitz, O. J.; Li, D.* An active derivatization detection method for inline monitoring the isolation of carbohydrates by preparative liquid chromatography. J Chromatogr A 2024, 1719, 464730.
(6) Li, X.; Ji, W.; Wu, S.; Qian, C.; Zhou, J.*; Zhang, Z.*; Li, D*. The isolation, characterization and biological activities of the non-glucan polysaccharides from the high-starch-content plant Pueraria mirifica. Int J Biol Macromol 2024, 261, 129709.
(7) Zhu, K.; Zhang, Z.; Li, G.; Sun, J.; Gu, T.; Ain, N. U.; Zhang, X.*; Li, D.* Extraction, structure, pharmacological activities and applications of polysaccharides and proteins isolated from snail mucus. Int J Biol Macromol 2024, 258, 128878.
(8) Yuan, S.; Zhang, M.; Yao, Z.; Liu, J.*; Li, X.; Zhang, Z.*; Li, D.* Isolation, structural characterization, and bioactivities of neutral polysaccharides from Zizania latifolia. Int J Biol Macromol 2024, 254, 127679.
2023年
(1) Yuan, S.*; Wang, J.; Li, X.; Zhu, X.; Zhang, Z.*; Li, D.* Study on the structure, antioxidant activity and degradation pattern of polysaccharides isolated from lotus seedpod. Carbohydr Polym 2023, 316, 121065.
(2) Huang, X.; Huang, X.; Li, Q.; Ma, M.; Cui, Y.; Yang, L.; Wang, H.; Luo, R.; Chen, J.; Yang, J.; Lin, J.; Li, D.*, Zheng Y.*; Zhang, J.* Seco-cyclic phorbol derivatives and their anti-HIV-1 activities. Chin J Nat Med 2023, 21, 1-12.
(3) 沙云菲; 熊骏威; 翟钰霖; 王宝雷; 钟志华; 费婷*; 李笃信*; 吴达. 基于捕集柱阵列的制备型二维液相色谱纯化烟草中的４个组分. 色谱 2023, 41 (3), 281-286.
2022年
(1) Zhai, Y.; Li, G.; Peng, K.; Ge, Z.; Zhang, W.; Li, D.* Less Configuration and More Dimensionality: Preparative Heart-Cut Multidimensional Liquid Chromatography Based on Trapping Arrays. Anal Chem 2022, 94 (49), 16997-17002.
(2) Yan, N.; Li, X.; Zhou, C.; Jiang, Q.; Li, J.; Zhang, Z.; Ouyang, Y.*; Li, D.*; Li, J.* Characterization of degradation products of carrageenan by LC-QTOF/MS with a hypothetical database. Food Chem 2022, 384, 132504.
(3) Li, D.; Qi, Z.; Liu, J.; Zhou, J.*. Effect of pH on the conformational structure of cytochrome c and subsequent enzymatic cross-linking catalyzed by laccase. Electron J Biotechnol 2022, 60, 1-10.
(4) Ain, N. U.; Wu, S.; Li, X.; Li, D.*; Zhang, Z.* Isolation, Characterization, Pharmacology and Biopolymer Applications of Licorice Polysaccharides: Review. Materials (Basel) 2022, 15 (10), 3654.
(5) Yan N, Song F, Ouyang Y, Li D, Tian H, Yi L, et al. Glycan Mapping of Low-Molecular-Weight Heparin Using Mass Spectral Correction Based on Chromatography Fitting with Glycomapping Software. Anal Chem. 2022;94(38):13000–9.
2021年
(1) Xu, S.; Li, Z.; Zhang, L.; Zhang, W.*; Li, D.* In situ growth of COF-rLZU1 on the surface of silica sphere as stationary phase for high performance liquid chromatography. Talanta 2021, 221, 121612.
2020年
(1) 朱梦; 李笃信*; 杨凯歌; 李富川; 张真庆*. 不同来源肝素酶Ⅰ对依诺肝素钠降解作用的研究. 药物分析杂志 2020, 40 (07), 1254-1261.
(2) Chu, Z.; Zhang, W.*; Li, D.*; Zhang, L.; Zhu, M.; Ge, Z. Synthesis and chromatographic evaluation of poly(pentabromostyrene)-silica composite: A versatile stationary phase for separating both polar and non-polar aromatic compounds. Microchem J 2020, 156, 104838.
长期从事液相色谱纯化工艺及仪器研究，提出了“少即是多”极简主义科学仪器设计理念，开发了制备型二维液相色谱系统和多糖纯化仪，开发了多种皮肤病治疗活性多糖成分，以第一作者或通讯作者在Anal Chem, J Chromatogr A, J Pharm Anal, Carbohydr Polym, 色谱等领域顶级期刊上，已发表论文36篇，以第一发明人申报发明专利23项，已授权发明专利9项，参与制定国家标准1项（GB/T 44739-2024）。获中国商业联合会科学技术奖二等奖1项和中国食品工业协会科学技术奖三等奖1项。
《中草药》《Pharm Sci Adv》等期刊青年编委、世界中联中药化学专业委员会理事、江苏省化学化工学会色谱及分离科学专业委员会委员、江苏省营养学会分子营养专业委员会委员、江苏省科技副总（苏州金记食品有限公司）。
1. 多维液相色谱分离纯化设备及其方法；
2. 多糖/寡糖的纯化及构效关系研究；
3. 多糖在药物、化妆品和保健食品中应用开发；
2026年
(1) Jiang X, Xia J, Gu T, Zhang M, Wang Y, Han H, et al. Structure and bioactivities of a glycan isolated from Fritillaria unibracteata. Int J Biol Macromol. 2026;357:151613.
(2) Xia J, Zohaib MA, Su X, Ji W, Hu T, Zhou J, et al. Uncovering a photoprotective polysaccharide from Gentiana dahurica via systematic fractionation. Pharmaceutical Science Advances. 2026;4:100120.
(3) Zhao L, Gao Z, Yuan Z, Yang X, Li D, Ren Q, et al. DC-CD4 bispecific tolerogenic nanovesicles induce antigen-specific regulatory T cells and ameliorate collagen-induced arthritis in mice. Nature Communications. 2026.
(4) Li S, Wang K, Shi W, Wang X, Li D, Liu Y, et al. Vm–MSI: a Vancomycin–Antimicrobial Peptide Conjugate Combating Resistant Bacteria and Broadening the Antimicrobial Spectrum. Advanced Science. 2026;13(11):e03023.
2025年
(1) Zhu, K.; Yao, Z.; Gu, T.; Jiang, X.; Zhou, J.*; Li, D.* Study of the ability of polysaccharides isolated from Zizania latifolia to promote wound healing in mice via in vitro screening and in vivo evaluation. Food Chem 2025, 464, 141810.
(2) Ain, N. U.; Akhlaq, M. H.; Zhu, K.; Ji, W.; Yi, L.*; Li, D.*; Zhang, Z.* A nanoemulgel formulation of acetylated Crataegus pinnatifida polysaccharide for the treatment of skin photoaging in mice. Int J Biol Macromol 2025, 303, 140649.
(3) Ji W, Nie X, Sun L, Wang Y, Tian S, Li D. Development of hydro-distillation extraction and macroporous resin enrichment method for GC-MS chemical profiling of volatile components in the dried root of Gentiana dahurica Fisch. Nat Prod Res. 2025;39(20):6016–22.
(4) Li G, Shi Y, Zhu J, Zhu K, Hu B, Huang X, et al. Active Peptides Derived from Snail Mucus Promoted Wound Healing by Enhancing Endothelial Cell Proliferation and Angiogenesis. International Journal of Molecular Sciences. 2025;26(21):10341.
(5) 王子梵, 姚振, 初红涛, 许展鹏, 李笃信, 张维冰. 基于溶剂诱导相变萃取法麻类植物中糖苷与苷元的分级研究. 齐齐哈尔大学学报(自然科学版). 2025(01):2–7.
(6) Zhang M, Zhao L, Xia J, Zhu X, Liu J, Sun L, et al. Exploring the hepatoprotective efficacy of Cortex Fraxini from the perspective of interplay between antibacterial molecule and the gut microbiota. Fitoterapia. 2025;185:106737.
2024年
(1) Wang J.; Zhu, K.; Zhang, M.; Zhou, Q.; Ji, W.; Yao, Z.; Li, D*. Pharmacokinetics, tissue distribution, and subacute toxicity of oral carrageenan in mice. Int J Biol Macromol 2024, 266, 130725.
(2) Li, D.; Du, X.; Bai, W.; Schmitz, O. J. Sample preparation of Hedyotis diffusa Willd. for two-dimensional gas chromatography-time of flight mass spectroscopic analysis enhanced chemical profiling. Pharm Sci Adv 2024, 2, 100056.
(3) Ain, N. U.; Khan, B.; Zhu, K.; Ji, W.; Tian, H.; Yu, X.; Yi, L.*; Li, D.*; Zhang, Z.* Fabrication of mesoporous silica nanoparticles for releasable delivery of licorice polysaccharide at the acne site in topical application. Carbohydr Polym 2024, 339, 122250.
(4) Ji, W.; Qian, C.; Su, X.; Li, X.; Zhang, Z.; Ma, Y.*; Zhang, M.*; Li, D.* Structure characterization and protective effect against UVB irradiation of polysaccharides isolated from the plateau plant Gentiana dahurica Fisch. Int J Biol Macromol 2024, 267, 131551.
(5) Yao, Z.; Zhu, K.; Gu, T.; Schmitz, O. J.; Li, D.* An active derivatization detection method for inline monitoring the isolation of carbohydrates by preparative liquid chromatography. J Chromatogr A 2024, 1719, 464730.
(6) Li, X.; Ji, W.; Wu, S.; Qian, C.; Zhou, J.*; Zhang, Z.*; Li, D*. The isolation, characterization and biological activities of the non-glucan polysaccharides from the high-starch-content plant Pueraria mirifica. Int J Biol Macromol 2024, 261, 129709.
(7) Zhu, K.; Zhang, Z.; Li, G.; Sun, J.; Gu, T.; Ain, N. U.; Zhang, X.*; Li, D.* Extraction, structure, pharmacological activities and applications of polysaccharides and proteins isolated from snail mucus. Int J Biol Macromol 2024, 258, 128878.
(8) Yuan, S.; Zhang, M.; Yao, Z.; Liu, J.*; Li, X.; Zhang, Z.*; Li, D.* Isolation, structural characterization, and bioactivities of neutral polysaccharides from Zizania latifolia. Int J Biol Macromol 2024, 254, 127679.
2023年
(1) Yuan, S.*; Wang, J.; Li, X.; Zhu, X.; Zhang, Z.*; Li, D.* Study on the structure, antioxidant activity and degradation pattern of polysaccharides isolated from lotus seedpod. Carbohydr Polym 2023, 316, 121065.
(2) Huang, X.; Huang, X.; Li, Q.; Ma, M.; Cui, Y.; Yang, L.; Wang, H.; Luo, R.; Chen, J.; Yang, J.; Lin, J.; Li, D.*, Zheng Y.*; Zhang, J.* Seco-cyclic phorbol derivatives and their anti-HIV-1 activities. Chin J Nat Med 2023, 21, 1-12.
(3) 沙云菲; 熊骏威; 翟钰霖; 王宝雷; 钟志华; 费婷*; 李笃信*; 吴达. 基于捕集柱阵列的制备型二维液相色谱纯化烟草中的４个组分. 色谱 2023, 41 (3), 281-286.
2022年
(1) Zhai, Y.; Li, G.; Peng, K.; Ge, Z.; Zhang, W.; Li, D.* Less Configuration and More Dimensionality: Preparative Heart-Cut Multidimensional Liquid Chromatography Based on Trapping Arrays. Anal Chem 2022, 94 (49), 16997-17002.
(2) Yan, N.; Li, X.; Zhou, C.; Jiang, Q.; Li, J.; Zhang, Z.; Ouyang, Y.*; Li, D.*; Li, J.* Characterization of degradation products of carrageenan by LC-QTOF/MS with a hypothetical database. Food Chem 2022, 384, 132504.
(3) Li, D.; Qi, Z.; Liu, J.; Zhou, J.*. Effect of pH on the conformational structure of cytochrome c and subsequent enzymatic cross-linking catalyzed by laccase. Electron J Biotechnol 2022, 60, 1-10.
(4) Ain, N. U.; Wu, S.; Li, X.; Li, D.*; Zhang, Z.* Isolation, Characterization, Pharmacology and Biopolymer Applications of Licorice Polysaccharides: Review. Materials (Basel) 2022, 15 (10), 3654.
(5) Yan N, Song F, Ouyang Y, Li D, Tian H, Yi L, et al. Glycan Mapping of Low-Molecular-Weight Heparin Using Mass Spectral Correction Based on Chromatography Fitting with Glycomapping Software. Anal Chem. 2022;94(38):13000–9.
2021年
(1) Xu, S.; Li, Z.; Zhang, L.; Zhang, W.*; Li, D.* In situ growth of COF-rLZU1 on the surface of silica sphere as stationary phase for high performance liquid chromatography. Talanta 2021, 221, 121612.
2020年
(1) 朱梦; 李笃信*; 杨凯歌; 李富川; 张真庆*. 不同来源肝素酶Ⅰ对依诺肝素钠降解作用的研究. 药物分析杂志 2020, 40 (07), 1254-1261.
(2) Chu, Z.; Zhang, W.*; Li, D.*; Zhang, L.; Zhu, M.; Ge, Z. Synthesis and chromatographic evaluation of poly(pentabromostyrene)-silica composite: A versatile stationary phase for separating both polar and non-polar aromatic compounds. Microchem J 2020, 156, 104838.