研究方向

应用冷冻电镜技术研究核酸蛋白质复合体结构；非编码RNA加工代谢和功能机理。

发表论文 (^#first author; *corresponding author)

1)N. Cao^#, J. Wang^#*, T. Deng^#, B. Fan, S. Su, J. Ma and H.-W. Wang (2025). Structural basis of endo-siRNA processing by Drosophila Dicer-2 and Loqs-PD. Nucleic Acids Research 53. 10.1093/nar/gkaf102.

2)T. Deng, S. Su, X. Yuan, J. He, Y. Huang, J. Ma*, J. Wang*(2023). Structural mechanism of R2D2 and Loqs-PD synergistic modulation on DmDcr-2 oligomers. Nature Communications14, 5228. 10.1038/s41467-023-40919-1.

3)P. Deng, S. Q. Tan, Q. Y. Yang, L. Fu, Y. Wu, H. Z. Zhu, L. Sun, Z. Bao, Y. Lin, Q. C. Zhang, H. Wang, J. Wang*, and J. G. Liu* (2023). Structural RNA components supervise the sequential DNA cleavage in R2 retrotransposon. Cell. 10.1016/j.cell.2023.05.032.

4)Ao Sun, C. P. Li, Z. Chen, S. Zhang, D. Y. Li, Y. Yang, L. Q. Li, Y. Zhao, K. Wang, Z. Li, J. Liu, S. Liu, J. Wang*, and J. G. Liu* (2023). The compact Caspi (Cas12l) 'bracelet' provides a unique structural platform for DNA manipulation. Cell Res 33, 229-244. 10.1038/s41422-022-00771-2.

5)S. Su^#, J. Wang^#, T. Deng, X. Yuan, J. He, N. Liu, X. Li, Y. Huang, H. W. Wang, and J. Ma (2022). Structural insights into dsRNA processing by Drosophila Dicer-2-Loqs-PD. Nature 607, 399-406. 10.1038/s41586-022-04911-x.

6)J. Wang^#, X. Song^#, D. Zhang, X. Chen, X. Li, Y. Sun, C. Li, Y. Song, Y. Ding, R. Ren, E. H. Harrington, L. A. Hu, W. Zhong, C. Xu, X. Huang, H. W. Wang, and Y. Ma (2020). Cryo-EM structures of PAC1 receptor reveal ligand binding mechanism. Cell Res 30, 436-445. 10.1038/s41422-020-0280-2.

7)W. Jin^#, J. Wang^#, C. P. Liu, H. W. Wang, and R. M. Xu (2020). Structural Basis for pri-miRNA Recognition by Drosha. Mol Cell78, 423-433 e5. 10.1016/j.molcel.2020.02.024.

8)J. Wang^#, J. Wang^#, M. Hu^#, S. Wu, J. Qi, G. Wang, Z. Han, Y. Qi, N. Gao, H. W. Wang, J. M. Zhou, and J. Chai (2019). Ligand-triggered allosteric ADP release primes a plant NLR complex. Science 364. 10.1126/science.aav5868.

9)J. Wang^#, M. Hu^#, J. Wang^#, J. Qi, Z. Han, G. Wang, Y. Qi, H. W. Wang, J. M. Zhou, and J. Chai (2019). Reconstitution and structure of a plant NLR resistosome conferring immunity. Science 364. 10.1126/science.aav5870.

10)X. Fan^#, J. Wang^#, X. Zhang, Z. Yang, J. C. Zhang, L. Zhao, H. L. Peng, J. Lei, and H. W. Wang (2019). Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution. Nature Communications 10, 2386. 10.1038/s41467-019-10368-w.

11)Z. Liu^#, J. Wang^#, H. Cheng^#, X. Ke^#, L. Sun, Q. C. Zhang, and H. W. Wang (2018). Cryo-EM Structure of Human Dicer and Its Complexes with a Pre-miRNA Substrate. Cell 173, 1191-1203 e12. 10.1016/j.cell.2018.03.080.

12)S. Liu^#, J. Wang^#, B. Song, X. Gong, H. Liu, Q. Hu, J. Zhang, Q. Li, J. Zheng, H. Wang, H. E. Xu, J. Li, and B. Wang (2023). Conformational Dynamics of the D53-D3-D14 Complex in Strigolactone Signaling. Plant Cell Physiol. 10.1093/pcp/pcad067.

13)S. Niu^#, J. Wang^#, B. Bai, L. Wu, A. Zheng, Q. Chen, P. Du, P. Han, Y. Zhang, Y. Jia, C. Qiao, J. Qi, W. X. Tian, H. W. Wang, Q. Wang, and G. F. Gao (2021). Molecular basis of cross-species ACE2 interactions with SARS-CoV-2-like viruses of pangolin origin. EMBO J 40, e107786. 10.15252/embj.2021107786.

14)Liu Nan^#, Zheng Liming^#, Xu Jie^#,Wang Jia^#, Hu Cuixia, Lan Jun, Zhang Xing, Zhang Jincan, Xu Kui, Cheng Hang, Yang Zi, Gao Xin, Wang Xinquan, Peng Hailin, Chen Yanan, and Wang Hong-Wei (2021). Reduced graphene oxide membrane as supporting film for high-resolution cryo-EM. Biophysics Reports7, 227-238. 10.52601/bpr.2021.210007.

15)K. Liu^#, S. Tan^#, S. Niu^#, J. Wang^#, L. Wu, H. Sun, Y. Zhang, X. Pan, X. Qu, P. Du, Y. Meng, Y. Jia, Q. Chen, C. Deng, J. Yan, H. W. Wang, Q. Wang, J. Qi, and G. F. Gao (2021). Cross-species recognition of SARS-CoV-2 to bat ACE2. Proc Natl Acad Sci U S A 118. 10.1073/pnas.2020216118.

16)L. Wu^#, Q. Chen^#, K. Liu^#, J. Wang^#, P. Han, Y. Zhang, Y. Hu, Y. Meng, X. Pan, C. Qiao, S. Tian, P. Du, H. Song, W. Shi, J. Qi, H. W. Wang, J. Yan, G. F. Gao, and Q. Wang (2020). Broad host range of SARS-CoV-2 and the molecular basis for SARS-CoV-2 binding to cat ACE2. Cell Discov 6, 68. 10.1038/s41421-020-00210-9.

17)Y. Liu^#, H. Hu^#, J. Wang^#, Q. Zhou, P. Wu, N. Yan, H. W. Wang, J. W. Wu, and L. Sun (2019). Cryo-EM structure of L-fucokinase/GDP-fucose pyrophosphorylase (FKP) in Bacteroides fragilis. Protein Cell 10, 365-369. 10.1007/s13238-018-0576-x.

18)H. Yang^#, J. Wang^#, M. Liu^#, X. Chen, M. Huang, D. Tan, M. Q. Dong, C. C. Wong, J. Wang, Y. Xu, and H. W. Wang (2016). 4.4 A Resolution Cryo-EM structure of human mTOR Complex 1.Protein Cell 7, 878-887. 10.1007/s13238-016-0346-6.

19)G. Qu^#, P. S. Kaushal^#, J. Wang^#, H. Shigematsu, C. L. Piazza, R. K. Agrawal, M. Belfort, and H. W. Wang (2016). Structure of a group II intron in complex with its reverse transcriptase. Nat Struct Mol Biol 23, 549-57. 10.1038/nsmb.3220.

20)J. Wang^#, W. Wang^#, W. Song, Z. Han, H. Zhang, J. Chai, H. Wang, and J. Wang (2015). An improved method for phasing crystal structures with low non-crystallographic symmetry using cryo-electron microscopy data. Protein Cell6, 919-23. 10.1007/s13238-015-0219-4.

21)W. Song^#, J. Wang^#, Z. Han, Y. Zhang, H. Zhang, W. Wang, J. Chang, B. Xia, S. Fan, D. Zhang, J. Wang, H. W. Wang, and J. Chai (2015). Structural basis for specific recognition of single-stranded RNA by Toll-like receptor 13. Nat Struct Mol Biol 22, 782-7. 10.1038/nsmb.3080.

22)J. Wang^#, and C. C. Yin (2013). A Zernike-moment-based non-local denoising filter for cryo-EM images. Science China-Life Sciences 56, 384-390. 10.1007/s11427-013-4467-3.

23)Z. Yang, J. J. Fan, J. Wang, X. Fan, Z. Ouyang, H. W. Wang and X. Y. Zhou (2024). Electrospray-assisted cryo-EM sample preparation to mitigate interfacial effects. Nature Methods 21. 10.1038/s41592-024-02247-0.

24)C. A. Tsuchida, S. Y. Zhang, M. S. Doost, Y. Q. Zhao, J. Wang, E. O'Brien, H. Fang, C. P. Li, D. Y. Li, Z. Y. Hai, J. Chuck, J. Brotzmann, A. Vartoumian, D. Burstein, X. W. Chen, E. Nogales, J. A. Doudna, and J. J. G. Liu (2022). Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity. Molecular Cell82, 1199-+. 10.1016/j.molcel.2022.02.002.

25)X. Wang, C. X. Hu, W. Ye, J. Wang, X. F. Dong, J. Xu, X. R. Li, M. F. Zhang, H. Y. Lu, F. L. Zhang, W. Wu, S. D. Dai, H. W. Wang, and Z. Z. Chen (2022). Structure of Rift Valley Fever Virus RNA-Dependent RNA Polymerase. Journal of Virology 96.

26)Y. Lu, N. Liu, Y. B. Liu, L. M. Zheng, J. H. Yang, J. Wang, X. Jia, Q. R. Zi, H. L. Peng, Y. Rao, and H. W. Wang (2022). Functionalized graphene grids with various charges for single-particle cryo-EM. Nature Communications 13. ARTN 671810.1038/s41467-022-34579-w.

27)R. Yan, R. Wang, B. Ju, J. Yu, Y. Zhang, N. Liu, J. Wang, Q. Zhang, P. Chen, B. Zhou, Y. Li, Y. Shen, S. Zhang, L. Tian, Y. Guo, L. Xia, X. Zhong, L. Cheng, X. Ge, J. Zhao, H. W. Wang, X. Wang, Z. Zhang, L. Zhang, and Q. Zhou (2021). Structural basis for bivalent binding and inhibition of SARS-CoV-2 infection by human potent neutralizing antibodies. Cell Res 31, 517-525. 10.1038/s41422-021-00487-9.

28)M. Huang, X. Zhang, G. A. Toh, Q. Gong, J. Wang, Z. Han, B. Wu, F. Zhong, and J. Chai (2021). Structural and biochemical mechanisms of NLRP1 inhibition by DPP9. Nature 592, 773-777. 10.1038/s41586-021-03320-w.

29)S. Ma, D. Lapin, L. Liu, Y. Sun, W. Song, X. Zhang, E. Logemann, D. Yu, J. Wang, J. Jirschitzka, Z. Han, P. Schulze-Lefert, J. E. Parker, and J. Chai (2020). Direct pathogen-induced assembly of an NLR immune receptor complex to form a holoenzyme. Science 370. 10.1126/science.abe3069.

30)C. Liu, J. Ma, J. Wang, H. Wang, and L. Zhang (2020). Cryo-EM Structure of a Bacterial Lipid Transporter YebT. J Mol Biol 432, 1008-1019. 10.1016/j. JMB.2019.12.008.

31)N. Liu, X. Dong, C. Hu, J. Zeng, J. Wang, J. Wang, H. W. Wang, and M. Belfort (2020). Exon and protein positioning in a pre-catalytic group II intron RNP primed for splicing. Nucleic Acids Res 48, 11185-11198. 10.1093/nar/gkaa773.

32)Y. Hu, B. A. Desimmie, H. C. Nguyen, S. J. Ziegler, T. C. Cheng, J. Chen, J. Wang, H. Wang, K. Zhang, V. K. Pathak, and Y. Xiong (2019). Structural basis of antagonism of human APOBEC3F by HIV-1 Vif. Nat Struct Mol Biol 26, 1176-1183. 10.1038/s41594-019-0343-6.

33)K. Mei, Y. Li, S. Wang, G. Shao, J. Wang, Y. Ding, G. Luo, P. Yue, J. J. Liu, X. Wang, M. Q. Dong, H. W. Wang, and W. Guo (2018). Cryo-EM structure of the exocyst complex. Nat Struct Mol Biol 25, 139-146. 10.1038/s41594-017-0016-2.

34)M. S. Ma, J. J. Liu, Y. Li, Y. W. Huang, N. Ta, Y. Chen, H. Fu, M. D. Ye, Y. H. Ding, W. J. Huang, J. Wang, M. Q. Dong, L. Yu, and H. W. Wang (2017). Cryo-EM structure and biochemical analysis reveal the basis of the functional difference between human PI3KC3-C1 and-C2. Cell Research 27, 989-1001. 10.1038/cr.2017.94.

35)Z. M. Liu, J. Wang, G. Li, and H. W. Wang (2015). Structure of precursor microRNA's terminal loop regulates human Dicer's dicing activity by switching DExH/D domain. Protein & Cell 6, 185-193. 10.1007/s13238-014-0124-2.

36)S. H. Chang, D. P. Sun, H. H. Liang, J. Wang, J. Li, L. Guo, X. L. Wang, C. C. Guan, B. M. Boruah, L. M. Yuan, F. Feng, M. R. Yang, L. L. Wang, Y. Wang, J. Wojdyla, L. J. Li, J. W. Wang, M. T. Wang, G. H. Cheng, H. W. Wang, and Y. F. Liu (2015). Cryo-EM Structure of Influenza Virus RNA Polymerase Complex at 4.3 angstrom Resolution. Molecular Cell 57, 925-935. 10.1016/j.molcel.2014.12.031.